Biohacker and tech millionaire Bryan Johnson has publicly detailed the vaginal microbiome results of his partner, Kate Tolo, claiming she ranks in the “top one percent of all vaginas” and acts as the female counterpart in his high-profile longevity experiment. The couple, who co-founded the health-optimization platform Blueprint in 2021, are investing $2 million annually in a project aiming to make Tolo “the most measured female in history” to advance scientific understanding of women’s health and aging.
Johnson Highlights ‘Exceptional’ Microbiome Results
Earlier this month, Johnson shared Tolo’s vaginal microbiome report on social media, announcing a “100/100 score.” He emphasized the dominance of a specific protective bacterium.
“Her sample is dominated by the single most protective bacterial species a vagina can host (Lactobacillus crispatus),”
Johnson wrote. He provided statistical context for the finding.
“Only about 25-30% of reproductive age women globally are L. crispatus-dominant, and ‘dominant’ usually means above 50%. Kate is at 98.7%.”
According to Johnson, the lab analysis found no sexually transmitted infections or other pathogenic bacteria. He outlined the clinical correlations associated with this specific microbiome profile.
“This is linked to lower risk of BV [bacterial vaginosis], UTIs, yeast infections, HPV persistence, HSV-2 [genital herpes] and HIV acquisition, preterm birth, and improved IVF outcomes.”
Tolo Advocates for Selective Period Product Use
Tolo, who appears in the Netflix documentary Don’t Die: The Man Who Wants to Live Forever, attributes her vaginal health in part to careful selection of menstrual products. During a March 2023 appearance on the Live Well Be Well with Sarah Ann Macklin podcast, she explained her reluctance to use tampons regularly.
“I just am skeptical that as we continue to get more data, we’ll just find more and more scary information,”
she said, referencing studies suggesting tampons may expose women to toxic chemicals. Tolo described her personal strategy, which prioritizes less invasive options.
“I oscillate between just pads, or I also do occasionally use tampons in moments where I just want the extra security.”
“Maybe I’m going to be out and about and I’m exercising, or I’m going to be in a situation where like I want more comfort and I’m not going to like bleed everywhere – then I’ll use a tampon.”
“I occasionally use cups as well. I kind of always felt a little uncomfortable about [using tampons].”
Despite finding pads uncomfortable, she views them as the lesser of two evils.
“I try and get like organic [pads],”
she added.
“I don’t have any kind of scent associated with them and all the like nasty, synthetic stuff that you can get in pads as well.”
Tolo’s approach centers on minimizing exposure to synthetic materials and chemicals.
Johnson’s Broader Longevity Protocol
The vaginal microbiome analysis is one component of Johnson’s extensive, public biohacking regimen aimed at reversing biological age. His experiments have included:
Penile Rejuvenation Efforts
Johnson has documented protocols involving semen analysis, blood flow testing, Botox injections, and shockwave therapy, claiming to have lowered his “erection age” by over half. He has drawn controversy for comparing his erection data with that of his teenage son.
Multigenerational Plasma Exchange
In 2023, Johnson, his then-17-year-old son, and his 70-year-old father participated in what he termed the “world’s first multigenerational plasma exchange.” Johnson received a liter of his son’s plasma but later reported “no benefits” and switched to total plasma exchange.
Psychedelic Exploration
Johnson has documented experiences with high-dose psilocybin (5g) and 5-MeO-DMT (27mg), describing the latter as leaving him “stunned beyond comprehension.”
Rigorous Morning Routine
Johnson recently detailed a morning protocol lasting four hours and 46 minutes.
“My morning routine is always evolving as we update protocols. This is what I’m experimenting with right now,”
he writes on his website. The routine includes waking at 5 a.m., oral hygiene, light exposure, breathwork, pre-workout nutrition, 90 minutes of exercise, sauna, red/NIR light therapy, focused shockwave therapy on joints, shower, hair growth serum, skincare, and breakfast.
While Johnson states he is not “suggesting immortality,” he aims to extend human healthspans to the point where “we stop thinking about lifespans.” Tolo’s participation as a measured counterpart represents a significant expansion of the Blueprint protocol to address female-specific biology and longevity markers.